Project

Part:BBa_K1314013:Experience

Designed by: Seak Chi U, Chan Wai Lun, CHIU, Yee Ting   Group: iGEM14_Hong_Kong-CUHK   (2014-10-07)

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Applications of BBa_K1314013

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UNIQ114c5eb919871053-partinfo-00000000-QINU

Ammonium repleted condition(left) versus nitrogen depleted condition(right) for constitutive RFP expression and nifH regulated RFP expression in A. vinelandii

Under high Ammonium level, there is strong expression of RFP under constitutive promoter. Under nitrogen depleted condition, there is no growth of A.vinelandii. This proves that the stable genome integration is successful. The mutated A.vinelandii has already lost nitrogenase gene, and thus no longer be diazotrophic.

EcoRI PstI double digestion checking our biobrick, it shows a band of insert with expected size of 5000 bp. UNIQ114c5eb919871053-partinfo-00000003-QINU